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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
12/09/2022 |
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Data da última atualização: |
12/04/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
ANGELO, P. C. da S.; PEREIRA, L. F. P.; SERA, G. H.; SETA, T. |
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Afiliação: |
PAULA CRISTINA DA SILVA ANGELO, CNPCa. |
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Título: |
Coffee microspore cultivation to attain doubled-haploid stable plantlets. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
INTERNATIONAL HORTICULTURAL CONGRESS, 31., 2022, Angers, França. |
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DOI: |
10.17660/ActaHortic.2023.1359.14 |
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Idioma: |
Inglês |
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Notas: |
E-poster. |
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Conteúdo: |
The aim of this study was to explore the potentials of progenies produced by the introgression of disease resistance from related Coffea species to C. arabica . In the IDR-Parana, Brazil, hybrids of C. arabica (tetraploid) x C. racemosa (diploid) were obtained. Self-pollination of some of these hybrids produced very mild yields, despite the backcros to C. arabica . In an attempt to regenerate stable double-haploids, anthers and isolated microspores were grown in vitro . It was assumed that chromosomes captured in a microspore after meiosis can acquire homo and/or homeologous stability faster in vitro than in vivo. C. arabica anthers served as control. Flowers were collected when the young microspores were uninucleated, and disinfected with 8% active chlorine. Microspores were extracted in 90 mM mannitol using a food mixer that worked for a few seconds, washed and centrifuged twice at 100xg and grown in modified N6 liquid medium (10 5 cells/mL in 35 mm diameter plates). Explants were grown continuously for six months on the same N6 medium containing 6.5 mg/L auxins, 1.0 mg/L cytokinins and 0.5 mg/L gibberellin (GA3), at 27 o C in the dark. Isolated microspores produced embryo-like structures or microcalli at very low frequencies (0.3 per plate). The anthers, on the other hand, produced embryogenic calli . One particular endogenous fungus infected 70% of the control anthers but 5% produced friable calli . Some of these were embryogenic, but arrested when compared to calli produced by the interspecific hybrid anthers. Regarding the hybrid anthers, 25% were infected by a same endogenous bacteria but 8% produced embryogenic tissue, with globular embryos multiplying and maturing simultaneously upon transfer to N6 medium without growth regulators. After 1.2 years, the photomorphogenesis has been induced under light, and auxin/cytokinin ratio = 2 plus GA3. MenosThe aim of this study was to explore the potentials of progenies produced by the introgression of disease resistance from related Coffea species to C. arabica . In the IDR-Parana, Brazil, hybrids of C. arabica (tetraploid) x C. racemosa (diploid) were obtained. Self-pollination of some of these hybrids produced very mild yields, despite the backcros to C. arabica . In an attempt to regenerate stable double-haploids, anthers and isolated microspores were grown in vitro . It was assumed that chromosomes captured in a microspore after meiosis can acquire homo and/or homeologous stability faster in vitro than in vivo. C. arabica anthers served as control. Flowers were collected when the young microspores were uninucleated, and disinfected with 8% active chlorine. Microspores were extracted in 90 mM mannitol using a food mixer that worked for a few seconds, washed and centrifuged twice at 100xg and grown in modified N6 liquid medium (10 5 cells/mL in 35 mm diameter plates). Explants were grown continuously for six months on the same N6 medium containing 6.5 mg/L auxins, 1.0 mg/L cytokinins and 0.5 mg/L gibberellin (GA3), at 27 o C in the dark. Isolated microspores produced embryo-like structures or microcalli at very low frequencies (0.3 per plate). The anthers, on the other hand, produced embryogenic calli . One particular endogenous fungus infected 70% of the control anthers but 5% produced friable calli . Some of these were embryogenic, but arrested when compared to calli prod... Mostrar Tudo |
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Thesagro: |
Café; Embriogénese; Pólen; Rubiaceae. |
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Thesaurus Nal: |
Embryogenesis; Pollen. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/249059/1/Coffee-microspore-cultivation.pdf
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Marc: |
LEADER 02577nam a2200241 a 4500
001 2146372
005 2023-04-12
008 2022 bl uuuu u00u1 u #d
024 7 $a10.17660/ActaHortic.2023.1359.14$2DOI
100 1 $aANGELO, P. C. da S.
245 $aCoffee microspore cultivation to attain doubled-haploid stable plantlets.$h[electronic resource]
260 $aINTERNATIONAL HORTICULTURAL CONGRESS, 31., 2022, Angers, França.$c2022
500 $aE-poster.
520 $aThe aim of this study was to explore the potentials of progenies produced by the introgression of disease resistance from related Coffea species to C. arabica . In the IDR-Parana, Brazil, hybrids of C. arabica (tetraploid) x C. racemosa (diploid) were obtained. Self-pollination of some of these hybrids produced very mild yields, despite the backcros to C. arabica . In an attempt to regenerate stable double-haploids, anthers and isolated microspores were grown in vitro . It was assumed that chromosomes captured in a microspore after meiosis can acquire homo and/or homeologous stability faster in vitro than in vivo. C. arabica anthers served as control. Flowers were collected when the young microspores were uninucleated, and disinfected with 8% active chlorine. Microspores were extracted in 90 mM mannitol using a food mixer that worked for a few seconds, washed and centrifuged twice at 100xg and grown in modified N6 liquid medium (10 5 cells/mL in 35 mm diameter plates). Explants were grown continuously for six months on the same N6 medium containing 6.5 mg/L auxins, 1.0 mg/L cytokinins and 0.5 mg/L gibberellin (GA3), at 27 o C in the dark. Isolated microspores produced embryo-like structures or microcalli at very low frequencies (0.3 per plate). The anthers, on the other hand, produced embryogenic calli . One particular endogenous fungus infected 70% of the control anthers but 5% produced friable calli . Some of these were embryogenic, but arrested when compared to calli produced by the interspecific hybrid anthers. Regarding the hybrid anthers, 25% were infected by a same endogenous bacteria but 8% produced embryogenic tissue, with globular embryos multiplying and maturing simultaneously upon transfer to N6 medium without growth regulators. After 1.2 years, the photomorphogenesis has been induced under light, and auxin/cytokinin ratio = 2 plus GA3.
650 $aEmbryogenesis
650 $aPollen
650 $aCafé
650 $aEmbriogénese
650 $aPólen
650 $aRubiaceae
700 1 $aPEREIRA, L. F. P.
700 1 $aSERA, G. H.
700 1 $aSETA, T.
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Registro original: |
Embrapa Café (CNPCa) |
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Biblioteca |
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Tipo/Formato |
Classificação |
Cutter |
Registro |
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URL |
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| Registros recuperados : 23 | |
| 1. |  | ANGELO, P. C. da S.; PEREIRA, L. F. P.; SERA, G. H.; SETA, T. Coffee microspore cultivation to attain doubled-haploid plantlets. Acta Horticulturae, v. 31, n. 1359, p. 123-130, 2023. Apresentado no INTERNATIONAL HORTICULTURAL CONGRESS, 31., 2022, Angers, França.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 4 |
| Biblioteca(s): Embrapa Café. |
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| 4. | | ANGELO, P. C. da S.; PEREIRA, L. F. P.; SERA, G. H.; CAIXETA, E. T. Some Coffea liberica SH3-LRR-coding sequences are highly distinguishable. Acta Horticulturae, v. 31, n. 1362, p. 283-287, 2023. Apresentado no INTERNATIONAL HORTICULTURAL CONGRESS, 31., 2022, Angers, França.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 4 |
| Biblioteca(s): Embrapa Café. |
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| 6. | | ANGELO, P. C. da S.; FERREIRA, I. B.; CARVALHO, C. H. S. de; MATIELLO, J. B.; SERA, G. H. Arabica coffee fruits phenology assessed through degree days, precipitation, and solar radiation exposure on a daily basis. International Journal of Biometeorology, v. 63, n. 7, p. 831-843, 2019| Tipo: Artigo em Periódico Indexado |
| Biblioteca(s): Embrapa Café. |
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| 7. | | ANGELO, P. C. da S.; ARIYOSHI, C.; CAIXETA, E. T.; PEREIRA, L. F. P.; RIBAS, A. F.; SERA, G. H. Variabilidade no domínio LRR de peptídeos codificados em "clusters" homólogos ao SH3 em diferentes genomas de Coffea. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 10., 2019, Vitória, ES. Pesquisa, inovação e sustentabilidade dos cafés do Brasil. Brasília, DF: Embrapa Café, 2019. Título em inglês: LRR domain variability for peptides encoded in gene clusters homologous to the SH3 in different Coffea genomes.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
|  |
| 8. | | ANGELO, P. C. da S.; CAIXETA, E. T.; PEREIRA, L. F. P.; RIBAS, A. F.; SERA, G. H. Os loci SH3 envolvidos na resistência à ferrugem são complexos, multialélicos e divergentes em genomas de coffea. Brasília, DF: Embrapa Café, 2022. 21 p. PDF. (Embrapa Café. Boletim de Pesquisa e Desenvolvimento, 3). Título em inglês: SH3 loci involved in the resistance to leaf rust are complex, multi allelic and divergent in coffea.| Tipo: Boletim de Pesquisa e Desenvolvimento |
| Biblioteca(s): Embrapa Café. |
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| 9. | | CHARMETANT, P.; PEREIRA, L. F.; SERA, G. H.; MARRACCINI, P.; ANDRADE, A. C.; MOUEN, J.; AUKOUMOU, M. M.; ANNEBIQUE, F.; LEROY, T. Adaptation of Coffea arabica F1 Hybrids to various environmental conditions. In: EUCARPIA GENETIC RESOURCES, 2017, Montpellier. Abstracts book... Montpellier: INRA, 2017.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 10. | | CARVALHO, C. H. S. de; BARTELEGA, L.; SERA, G. H.; MATIELLO, J. B.; ALMEIDA, S. R. de; SANTINATO, F.; HOTZ, A. L. Catálogo de cultivares de café arábica. Brasília, DF: Embrapa Café, 2022. 115 p. PDF. (Embrapa Café. Documentos, 16).| Biblioteca(s): Embrapa Café. |
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| 11. | | GASPARINI, A. K. dos S.; CINTRA, L. A.; CARDUCCI, F. C.; SHIGUEOKA, L. H.; SERA, G. H.; SERA, T.; ANGELO, P. C. da S. Indução in vivo de poliploidia em Coffea canephora resistente a nematoides. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA PARA O PROGRESSO DA CIÊNCIA, 73., 2021, Juiz de Fora, MG. Anais... Juiz de Fora, MG: SBPC, 2021. 4 p.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 12. | | SERA, G. H.; CARVALHO, C. H. S. de; ABRAHÃO, J. C. de R.; POZZA, E. A.; MATIELLO, J. B.; ALMEIDA, S. R. de; BARTELEGA, L.; BOTELHO, D. M. dos S. Coffee leaf rust in Brazil: historical events, current situation, and control measures. Agronomy, v. 12, n. 2, 2022. 19 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Café. |
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| 13. | | ARIYOSHI, C.; SERA, G. H.; RODRIGUES, L. M. R.; CARVALHO, F. G.; SHIGUEOKA, L. H.; MENDONÇA, A. E. S.; PEREIRA, C. T. M.; DESTÉFANO, S. A. L.; PEREIRA, L. F. P. Development and validation of an allele-specific marker for resistance to bacterial halo blight in coffea arabica. Agronomy, v. 12, n. 12, 3178, 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Café. |
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| 14. | | ALMEIDA, S. F.; SANTOS, M. F. A.; STEFANELO, D.; MATTOS, V. S.; RODRIGUES-SILVA, P. L.; SOUSA, G. P.; SALGADO, S. M. L.; SERA, G. H.; CASTAGNONE-SERENO, P.; CARES, J. E.; CARNEIRO, R. M. D. G. Genetic variability and phylogenetic characterization of different populations of Meloidogyne izalcoensis and reaction of coffee genotypes to this new species detected in Brazil. Plant Pathology, v. 72, n. 3, p. 636-650, 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 15. | | ANGELO, P. C. da S.; YAMAGISHI, M. E. B.; SILVA, A. G.; PONCE, T. P.; ARIYOSHI, C.; SHIGUEOKA, L. H.; ARANTES, T.; GASPARINI, A. K.; SERA, G. H.; PEREIRA, L. F. P.; CAIXETA, E. T. Could genes allocated to SH3 loci contribute to other resistance factors? In: ASIC CONFERENCE ON COFFEE SCIENCE, 29., 2023, Hanoi, Vietnam. Books of abstracts... Montpellier: ASIC, 2023. p. 167. ASIC 2023.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Café. |
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| 16. | | YUYAMA, P. M.; POT, D.; DEREEPER, A.; POINTET, S.; SILVA, J. B. G. D. da; SERA, G. H.; SERA, T.; CHARMETANT, P.; DOMINGUES, D. S.; LEROY, T.; PEREIRA, L. F. P. Identificação de polimorfismos em genótipos de Coffea arabica de uma coleção da Etiópia. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 8., 2013, Salvador. Sustentabilidade e inclusão Social. Brasília, DF: Embrapa Café, 2013.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 17. | | OLIVEIRA, D. de; CARAMORI, P. H.; RICCE, W. da S.; COSTA, A. B. da; SERA, G. H. S.; SEIXAS, C. D. S.; OKUYAMA, L. A.; MACHADO, J.; CAMPOS, L. A. C.; RIEDE, C. R.; BASSOI, M. C. Nota técnica: excesso de chuvas causa perdas na safra de trigo. Londrina: IAPAR, 2009. 11 p.| Tipo: Comunicado Técnico/Recomendações Técnicas |
| Biblioteca(s): Embrapa Soja. |
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| 18. | | SILVA, B. S. R. da; SANT'ANA, G. C.; CHAVES, C. L.; ANDROCIOLI, L. G.; FERREIRA, R. V.; SERA, G. H.; CHARMETANT, P.; LEROY, T.; POT, D.; DOMINGUES, D. S.; PEREIRA, L. F. P. Population structure and genetic relationships between Ethiopian and Brazilian Coffea arabica genotypes revealed by SSR markers. Genetica, v. 147, n. 2, p. 205–216, April 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Café. |
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| 19. | | ARIYOSHI, C.; SANT'ANA, G. C.; FELICIO, M. S.; SERA, G. H.; NOGUEIRA, L. M.; RODRIGUES, L. M. R.; FERREIRA, R. V.; SILVA, B. S. R. da; RESENDE, M. L. V. de; DESTÉFANO, S. A. L.; DOMINGUES, D. S.; PEREIRA, L. F. P. Genome-wide association study for resistance to Pseudomonas syringae pv. garcae in Coffea arabica. Frontiers in Plant Science, v. 13, article 989847, 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Café. |
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| 20. | | SANT'ANA, G. C.; PEREIRA, L. F. P.; POT, D.; IVAMOTO, S. T.; DOMINGUES, D. S.; FERREIRA, R. V.; PAGIATTO, N. F.; SILVA, B. S. R. da; NOGUEIRA, L. M.; KITZBERGER, C. S. G.; SCHOLZ, M. B. S.; OLIVEIRA, F. F. de; SERA, G. H.; PADILHA, L.; LABOUISSE, J-P.; GUYOT, R.; CHARMETANT, P.; LEROY, T. Genome-wide association study reveals candidate genes influencing lipids and diterpenes contents in Coffea arabica L . Scientific Reports, v. 8, n. 465, p. 1-12, 2018| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Café. |
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| Registros recuperados : 23 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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